PROBE SEQUENCE DETERMINATION SYSTEM FOR DNA ARRAY

摘要

PROBLEM TO BE SOLVED: To realize a probe sequence without cross-hybridization, having an equalized Tm and slightly assuming a secondary structure in order to assure the assay accuracy and reproducibility as a probe sequence for a DNA array. SOLUTION: The probe sequence determination for the DNA array is carried out by passing the probe design flow through the following at least two steps. A step of preparing a probe candidate list for the DNA probe array composed of a partial sequence of the genome or a cDNA sequence (a listing step) and a step of excluding candidates having the melting temperature deviating from the predetermined range or high stability of the secondary structure or possibility of cross-hybridization (a filtering step). Thereby, a method for designing the sequence of the probe DNA immobilized on the DNA probe array is provided.