TYROSYL−TRNA SYNTHASE VARIANTS

摘要

Tyrosyl−tRNA synthases having a modified amino acid sequence whereby unnatural amino acids can be more efficiently incorporated than original natural amino acids. More specifically, tyrosyl−tRNA synthases having been modified at the amino acid(s) at the 37− and/or 195−positions. A method of modifying tyrosyl−tRNA synthase characterized in that the position of an amino acid to be modified is determined based on the 3−D structure of an ANP complex of tyrosyl synthase and an amino acid to which this enzyme binds.