METHOD FOR PRODUCING A MARKER-FREE MUTATED TARGET ORGANISM AND PLASMID VECTORS SUITABLE FOR THE SAME

摘要

The invention relates to a plasmid vector which does not replicate in a targ et organism and contains the following constituents: a) an origin of replicatio n for a host organism which is not identical to the target organism, b) at lea st one genetic marker, c) optionally a section of a sequence which enables the transfer of DNA by means of conjugation (mob sequence), d) a section of a sequence which is homologous to sequences of the target organism and enables homologous recombination in the target organism, e) a gene for a galactokina se under the control of a promoter.