METHODS OF IDENTIFYING FUNCTIONAL ANALOGS OF PEPTIDE REGULATORS OF BIOLOGICAL PATHWAYS

摘要

A method of uncovering a putative functional analog of a peptide regulator of a biological pathway is disclosed. The method comprises: (a) generating a library including molecules representing: (i) constituents of the biological pathway; and/or (ii) portions of said constituents of the biological pathway; (b) contacting said molecules of said library with the peptide regulator to thereby obtain a complex composed of a molecule of said molecules of said library and the peptide regulator; (c) incubating said molecule and the peptide regulator of said complex in the presence of each of a plurality of distinct substances; and (d) identifying a substance of said plurality of distinct substances capable of competing with the peptide regulator for binding of said molecule to thereby uncover the putative functional analog of the peptide regulator of the biological pathway.