METHOD FOR PURIFICATION OF VIRAL VECTORS HAVING PROTEINS WHICH BIND SIALIC ACID

摘要

A method for isolating, from a mixture, a virus having a surface protein wit h a binding site for sialic acid is provided. The method involves contacting t he mixture with mucin which has been linked to a solid support and washing the solid support to remove material from the mixture which is non-specifically bound to the mucin- linked support. Thereafter, the specifically bound virus (e.g., AAV4 or AAV5) may be removed in a further washing step utilizing a concentrated salt or solution with low pH. Also described are pharmaceutical kits containing solid supports linked to mucin for use in isolating virus having a surface protein with a binding site for sialic acid, or detecting t he presence of the virus in a biological sample.