发明名称 |
Pharmacological targeting of bacterial DNA ligase for treatment and prevention of bacterial infections |
摘要 |
This invention provides methods for the discovery of molecules that target an essential step of bacterial DNA replication-the sealing of DNA strands by NAD+-dependent DNA ligase. Specific structural components of NAD+-dependent DNA ligase that are important for the reaction of DNA ligase with NAD+ and that comprise a putative binding site for the NMN component of NAD+ were identified. The invention also includes recombinant DNA ligase enzymes that are defective in their reaction with NAD+, but active in the ligation of pre-adenylated DNA nicks. An underlying principle of this invention is the use of NAD+-reactive and NAD+-defective ligases to identify molecules that specifically bind to the NAD+-binding site of DNA ligase and thereby interfere with the reaction of DNA ligase with NAD+.
|
申请公布号 |
US2003036647(A1) |
申请公布日期 |
2003.02.20 |
申请号 |
US20020179784 |
申请日期 |
2002.06.24 |
申请人 |
SHUMAN STEWART;SRISKANDA VERL |
发明人 |
SHUMAN STEWART;SRISKANDA VERL |
分类号 |
C12N9/00;(IPC1-7):C07H21/04;C12N9/22 |
主分类号 |
C12N9/00 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|