| 摘要 |
<p>It is intended to construct a high-performance specific gene amplification primer for detecting, quantifying and identifying enteritis vibrio which has practically satisfactory amplification efficiency and amplification specificity and shows a low possibility of judgment error. The base sequences of RNA polymerase σ70 factor rpoD genes of a standard strain of Vibrio and enteritis vibrio stock cultures (a toxin gene-containing strain and a toxin gene-free strain) are determined and the systemic relationships among them are clarified. Thus, bases characteristic to enteritis vibrio are identified and a highly specific probes having these bases and a gene amplification primer having high specificity and high amplification efficiency can be designed.</p> |
