| 摘要 |
<p>A diffraction grating type sensor chip for sample analysis using the surface plasmon resonance (SPR), and an analysis method and an analysis apparatus using the sensor chip. The sensor chip (1) has a plurality of diffraction grating surfaces (5a to 5i) in the vicinity of a metal layer (3). Groove pitch and groove direction of the diffraction grating surfaces (5a to 5i) and arrangement angle with respect to a predetermined reference plane (S0) are so determined that the groove direction on a predetermined projection plane is identical and the groove pitch is different from each other. Thus, even when parallel light is applied, intensity of reflected light from the diffraction grating surfaces (5a to 5i) has differences in two-dimensional distribution. Accordingly, for the entire sensor surface, it is possible to calculate at real time a resonance angle according to the intensity of the reflected light from the diffraction grating surfaces (5a to 5i) and the substantial incident angle to the diffraction grating surfaces (5a to 5i). Moreover, when performing analysis according to an intensity change amount of the reflected light, it is possible to substantially increase the measurement range.</p> |
