PHENOTYPIC EFFECTS OF UBIQUINONE DEFICIENCIES AND METHODS OFSCREENING THEREOF

摘要

The present invention relates to a method of screening for a compound allowi ng survival of clk1 homozygous mutant embryos; a method of screening for a compound suitable for rescue of mutant phenotype of mclk1 homozygous cell line; a method of screening for a compound suitable for partial or complete functional replacement of endogenous ubiquinone; a method for screening a compound capable of inhibiting activity of clk-1 and/or other processes required to make ubiquinone from demethoxyubiquinone; a non-ubiquinone- producer mouse; a DNA construct, which comprises an alteration of mclk1; a n on- ubiquinone-producer ES cell line; a coq-3 mutant subject non-ubiquinone producer; a method of screening for a compound suitable for complete or partial functional ubiquinone or demethoxyubiquinone replacement; a method f or reducing and/or increasing ubiquinone level in a multicellular subject; a method of screening for a genetic suppressor of clk-1; and a method of screening for a genetic suppressor of coq-3.