METHOD OF CONSTRUCTING POLYNUCLEOTIDE MICROARRAY, APPARATUS FOR THE CONSTRUCTION AND POLYNUCLEOTIDE MICROARRAY

摘要

An object of the present invention is to provide a method for preparing a polynucleotide microarray which enables fixation of a sufficient amount of polynucleotides onto the support and can provide signals having uniform and sufficient intensity within a spot area, even when the polynucleotides are synthetic oligo polynucleotides which have a short chain length and are known to be difficult to be fixed by electrostatic binding. The present invention provides a method for preparing a polynucleotide microarray, wherein a solution for dissolving polynucleotides which is used to fix the polynucleotides onto a support contains substantially no anionic electrolyte, and is a solution of water or mixture of water and an organic solvent; and when the solution is a mixture of water and an organic solvent, the content of the organic solvent in the solution is less than 50 volume %.