METHOD FOR BREEDING MICROORGANISMIC CELL

摘要

PROBLEM TO BE SOLVED: To develop a gene modification process and a genome modification process high in general-purpose properties rapidly and certainly. SOLUTION: A host having the genome into which both of terminal sequences of an insertion sequence to be transduced are preliminarily inserted is transformed with a DNA bearing the insertion sequence, and a host microorganism is transformed due to the expression of the insertion sequence to be transduced. Then, the cells of the microorganism are bred in the transformed host cells.