Protein-protein interaction map inference using interacting domain profile pairs

摘要

Disclosed is a technique to predict protein-protein interaction maps across organisms called an "interacting-domain profile pair" method. The method uses a high-quality protein interaction map with interaction domain information as input to predict an interaction map in another organism. It combines sequence similarity searches with clustering based on interaction patterns and interaction domain information. Results can be compared with predictions from a naive inference method based only on full-length protein sequence similarity. This domain-based method can eliminate a significant amount of false-positives compared to methods that are the consequences of multi-domain proteins; as well as increase the sensitivity by identifying new potential interactions.