| 摘要 |
<p>Provided are methods and materials for detecting, in a sample, the activity of an antibiotic which affects cell integrity, which methods comprise the steps of: (a) providing a transformed Streptomyces coelicolor microorganism which comprises a nucleic acid encoding a promoter derived from a gene of the vanSREFHAX cluster (e.g. from vanE = (SC66T3.03); vanF = (SC66T3.04); vanH (SC66T3.05), or vanR (SCH66.11c) of Streptomyces coelicolor operably linked to a heterologous reporter gene capable of causing a detectable signal, (b) contacting the sample with the transformed microorganism, (c) observing said transformed microorganism for said detectable signal. Also provided are processes for producing microorganisms for use in the method, and associated kits.</p> |
