METHOD AND DEVICE FOR MONITORING CELL MOTILITY IN REAL-TIME

摘要

The invention relates to devices, devices for arraying biomolecules, including cells, methods for arraying biomolecules, assays for monitoring cellular movement, and systems for monitoring cellular movement. The devices include a support (140), a first layer (150) configured to be placed in fluid-tight contact with the support, the first layer (150) having an upper surface (140a) and defining a pattern of micro-orifices (300), each micro-orifice of the pattern of micro-orifices (300) having walls (150a) and defining a micro-region on the support (140) when the first layer (150) is placed in fluid-tight contact with the support such that the walls (150a) of each micro-orifice (300) and the micro-region on the support (140) together define a micro-well (141); and a second layer (160) configured to be placed in fluid-tight contact with the upper surface of the first layer (150), the second layer (160) defining a pattern of macro-orifices (170), each macro-orifice (170) of the pattern of macro-orifices (170) having walls and defining a macro-region when the first layer is placed in fluid-tight contact with the support (140) and the second layer (160) is placed in fluid-tight contact with the first layer (150) such that the walls of the macro-orifice and the macro-region together define a macro-well (151).