| 摘要 |
<p>The present invention relates to a method for the detection of intermolecular interactions between probe(s) and protein targets, said targets including at least one protein or polypeptide, which, when expressed in vivo into host cells, is toxic for said host cells or cannot be purified, or does not fold correctly. In particular, the invention results from the combination of the preparation of protein arrays with cell-free synthesized proteins and the detection of specific interactions using infra-red fluorescent dyes. The invention also relates to protein arrays and methods for their preparation. A cell-free method for protein synthesis involves the addition of purified alpha subunit of RNA polymerase. An array of crude cells extracts (with non-purified proteins) is good enough for the detection of DNA-protein and protein-protein interactions.</p> |
