摘要 |
<p>The present invention provides a process for the isolation of betulinic acid by extracting rhizomes of Nelumbo nucifera Gaertn (Family - Nymphaeaceae) with a polar solvent by conventional soxhlet extraction methods, concentrating the extract, adding water to the concentrated residue, extracting the said residue successively with non-polar solvents, extracting the aqueous phase with organic solvent, concentrating the above obtained organic solvent phase and purifying the product by conventional methods such as column chromatography followed by crystallization to give pure betulinic acid.</p> |