| 摘要 |
PROBLEM TO BE SOLVED: To provide an anterior eye part-associated cell sheet or a three- dimensional structure by which a cell, a desmosome structure between the cells, and a basilar membrane-like protein between the cells and substrates are recovered in a maintained state. SOLUTION: The anterior eye part-associated cell sheet or the three- dimensional structure are produced by culturing the cells on a cell-culturing carrier having the surface of the substrate covered with a temperature- responsible polymer having 0-80 deg.C upper-limit or lower-limit critical solubilization temperature in water, optionally superposing the cultured cell layers, and thereafter, (1) regulating the temperature of the culturing liquid so as to be not lower than the upper-limit critical solubilization temperature or not higher than the lower-limit critical solubilization temperature, (2) optionally sticking the cultured anterior eye part-associated cell sheet or three-dimensional structure to a polymer membrane, and (3) peeling the anterior eye part-associated cell sheet or the three-dimensional structure with the polymer membrane as it is. |
