发明名称 |
Interleukin-3 (IL-3) mutant polypeptides |
摘要 |
<p>The present invention relates to recombinant human interleukin-3 (hIL-3) variant or mutant proteins (muteins). These hIL-3 muteins contain one or more amino acid substitutions and may also have amino acid deletions at both the N- and C- termini. The invention also relates to pharmaceutical compositions containing the hIL-3 muteins and methods for using them. Additionally, the present invention relates to recombinant expression vectors comprising nucleotide sequences encoding the hIL-3 muteins, related microbial expression systems, and processes for making the hIL-3 muteins using the microbial expression systems. <??>Included in the present invention are deletion mutants of hIL-3 in which from 1 to 14 amino acids have been deleted from the N-terminus, and from 1 to 15 amino acids (corresponding to residues 119 to 133) have been deleted from the C-terminus, and which also contain one to three amino acid substitutions in the polypeptide. These hIL-3 mutant polypeptides may have biological activities similar to or better than hIL-3 and, in some cases, may also have an improved side effect profile.</p> |
申请公布号 |
EP1283264(A2) |
申请公布日期 |
2003.02.12 |
申请号 |
EP20020021264 |
申请日期 |
1993.11.22 |
申请人 |
G.D. SEARLE LLC |
发明人 |
BAUER, S. CHRISTOPHER;ABRAMS, MARK ALLEN;BRAFORD-GOLDBERG, SARAH RUTH;CAPARON, MAIRE HELENA;EASTON, ALAN MICHAEL;KLEIN, BARBARA KURE;MCKEARN, JOHN PATRICK;OLINS, PETER O.;PAIK, KUMMAN;POLAZZI, JOSEPH O.;THOMAS, JOHN WARREN |
分类号 |
C12N15/09;A61K38/00;A61K38/20;A61K45/00;A61P7/00;A61P43/00;C07H21/04;C07K1/00;C07K14/00;C07K14/54;C07K17/00;C12N1/15;C12N1/19;C12N1/21;C12N5/08;C12N5/10;C12N15/24;C12N15/61;C12P21/02;C12P21/04;C12R1/19;(IPC1-7):C12N15/24 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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