The present invention discloses methods for identifying and analysing microsatellite-associated polymorphisms between different DNA samples. Different DNA samples, e.g. from different individuals, are analysed using a PCR based on the combination of a RAMP-primer and an AFLP-primer and polymorphisms between the different DNA samples are identified. The polymorphisms thus identified may be isolated and further analysed by e.g. DNA sequence analysis both upstream and downstream from the microsatellite-associated polymorphism. These sequences may subsequently be used to devise and synthesise new means for analysis of the polymorphic locus, such as e.g. PCR-primer pairs or oligonucleotide probes.
申请公布号
EP1282729(A2)
申请公布日期
2003.02.12
申请号
EP20010932397
申请日期
2001.05.15
申请人
KEYGENE N.V.
发明人
VAN EIJK, MICHAEL, JOSEPHUS, THERESIA;PELEMAN, JOHAN, DOMINICUS;DE RUITER-BLEEKER, MARIA, JOHANNA