BACILLUS TRANSFORMATION, TRANSFORMANTS AND MUTANT LIBRARIES
摘要
The present invention provides methods for building DNA constructs in vitro , transforming such constructs into competent Bacillus strains with good efficiency, and generating populations of mutants. Also provided is a method to assemble DNA constructs in situ .
申请公布号
WO0214490(A3)
申请公布日期
2003.02.06
申请号
WO2001US25166
申请日期
2001.08.10
申请人
GENENCOR INTERNATIONAL, INC.
发明人
DIAZ-TORRES, MARIA, R.;SCHELLENBERGER, VOLKER;SELIFONOVA, OLGA, V.;MORRISON, THOMAS, B.;LEE, EDWIN, W.