发明名称 |
Method for generating immortal dendritic cell lines |
摘要 |
Immortalized mammalian dendritic cell lines are provided that have characteristics of functional, mature dendritic cells; defined by surface phenotype, constitutive and inducible gene expression patterns, and functional effects on T lymphocytes. The cell lines are capable of continuous in vitro proliferation for extended periods of time. Of particular interest is the ability of the cell lines to present antigen in a stimulatory manner to naïve T cells. The dendritic cell lines are useful in gene discovery, the generation of dendritic cell subset-specific antibodies and probes; defining stages of dendritic cell differentiation and maturation; for induction of antigen specific stimulation of lymphocytes; for dissection of antigen specific anergy or tolerance in lymphocytes; for characterization of dendritic cell-natural killer cell interactions; for in vitro and in vivo analyses of dendritic cell-endothelial cell interactions; and for screening assays.
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申请公布号 |
US2003027334(A1) |
申请公布日期 |
2003.02.06 |
申请号 |
US20020121148 |
申请日期 |
2002.04.10 |
申请人 |
FITZPATRICK DAVID R.;ROUX EILEEN R.;MALISZEWSKI CHARLES R.;PESCHON JACQUES J. |
发明人 |
FITZPATRICK DAVID R.;ROUX EILEEN R.;MALISZEWSKI CHARLES R.;PESCHON JACQUES J. |
分类号 |
A01K67/027;A61K35/12;C12N5/0784;C12N15/85;G01N33/50;(IPC1-7):C12N5/08;C12N15/86;A61K48/00 |
主分类号 |
A01K67/027 |
代理机构 |
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