IMPROVED CAPTURE AND DETECTION OF TARGET NUCLEIC ACID IN DIPSTICK ASSAYS
摘要
Use of helper probes in dipstick assays is described. In a dipstick assay to test for the presence of a target nucleic acid in a sample solution, the sample solution is connected with the contact end of the dipstick to cause the sample solution is contacted with the contact end of the dipstick to cause the sample solution to move by capillary action to a capture zone of the dipstick at which target nucleic acid is captured. The target nucleic acid may be captured at the capture zone by a capture probe capable of hybridising to the target nucleic acid. A labelled detection probe capable of hybridising to the target nucleic acid may be used to detect the target nucleic acid at the capture zone. A helper probe may be used to enhance the binding of the capture and/or detection probe to the target nucleic acid, thereby improving the sensitivity of target nucleic acid detection. Dipsticks and kits are also described.
申请公布号
WO0204668(A3)
申请公布日期
2003.02.06
申请号
WO2001GB03024
申请日期
2001.07.06
申请人
LEE, HELEN;DINEVA, MAGDA, ANASTASSOVA;HAZELWOOD, SHAUN, CHRISTOPHER
发明人
LEE, HELEN;DINEVA, MAGDA, ANASTASSOVA;HAZELWOOD, SHAUN, CHRISTOPHER