Method of detecting sequence variations in macromolecules by thermal gradiant

摘要

The present invention describes a method for separating macromolecules, particularly heteroduplex from homoduplex DNA molecules. The macromolecules undergo a temperature sensitive structural denaturation which, when it occurs, results in a difference in the mobilities of the denatured components on a solid support. A polar to non-polar solvent gradient or modified solid support particular to each sample is not required, since the difference in mobility is achieved on conventional solid supports using a single mobile phase composition.