Human myoblast cell lines and their uses

摘要

Described is a human muscular cell line which is able to proliferate and generated from primary human muscular cells by a process comprising the step of:a) pre-treating a culture of said primary human muscular cells or a suspension thereof with at least one glucocorticoid,b) optional step comprising obtaining a suspension of said pre-treated culture of step a),c) transferring into the pre-treated cells of the suspension of step a) or b) at least one nucleic acid vector which is not of retroviral origin and which is competent to immortalize said pre-treated cells andd) culturing the transferred cells of step c).Furthermore, human muscular cells derived from said cell lines are provided as well as pharmaceutical and diagnostic compositions comprising the same.