METHOD FOR THE PREPARATION OF PLEUROMUTILINE AND ITS SULPHOESTERS

摘要

By the method pleuromutiline and its sulphoesters are produced by deep fermentation with producer strain Clitopilus pinsitus CBS - 62370. The compounds can be used in the pharmaceutical industry and in veterinary medicine for the treatment of a wide range of infectious diseases The advantages of the method are that it rules out the chance of partial hydrolysis of the glycol residue in C14 in the pleuromutiline molecule, the desired products being produced with high yield and HPLC purity. The cultivation, maintenance and the storage of the strain is held in a medium of malt agar with subsequent preparation of laboratory and production inoculum. There follows biosynthesis, triple extraction with C1-C3 alkanols, evaporation to dry, interaction with sulphochlorides in the presence of imidazole and subsequent sulphoester crystallization. 4 claims