New peptide that binds to protein phosphatase holoenzyme, and that is a fragment of a viral, parasite or cellular protein, useful e.g. for treating cancer, human immunodeficiency virus (HIV) and malaria

摘要

A peptide (I), shorter than 30, preferably 20, amino acids long that binds specifically, in vitro, to a type 2A protein phosphatase holoenzyme (II), is new. A new peptide (I), is shorter than 30, preferably 20, amino acids (aa) in length and binds specifically, in vitro, to a type 2A protein phosphatase (PP2A) holoenzyme (II). (I) is: (i) a fragment of a viral, parasite or cellular protein, i.e. t antigen of simian virus (SV)40 or polyoma, a medium t antigen of polyoma, a subunit of type B (Bm B' or B'') of PP2A, CK alpha 2, CaMIV, p70S6 kinase, Pak1/Pak3, Tap42/ alpha 4, PTPA, Set/I1/I2-PPA2, E4orf4, tau, cluster of differentiation (CD)28 or Vpr; or (ii) a variant of (i) having aa deleted or substituted provided it retains ability to bind to (II) or one of its subunits. Independent claims are also included for the following: (1) polypeptides comprising repeats of (I); (2) polynucleotides (III) that encode (I); (3) an expression vector containing (III) and expression control elements; (4) purified mono- or poly-clonal antibodies (Ab) that can bind specifically to (I); (5) identifying (I); and (6) producing (I).