发明名称 |
METHOD FOR DETECTING MUTATIONS IN NUCLEOTIDE SEQUENCES |
摘要 |
The invention relates to a method for simultaneously detecting mutations in different nucleotide sequences and for determining the transcription rate of mutated and non-mutated nucleotide sequences. The inventive method comprises the following steps: hybridizing single-stranded sample nucleotide sequences to single-stranded reference nucleotide sequences, fixating, before or during hybridization, single-stranded reference nucleotide sequences or single-stranded sample nucleotide sequences, or fixating, after or during hybridization, heteroduplices from reference and sample nucleotide sequences on an electronically addressable surface, incubating them with a substrate that recognizes heteroduplex mismatches, and detecting the substrate bonds. |
申请公布号 |
WO0212553(A3) |
申请公布日期 |
2003.01.30 |
申请号 |
WO2001EP08127 |
申请日期 |
2001.07.13 |
申请人 |
NANOGEN RECOGNOMICS GMBH;KAPPEL, ANDREAS;POLAKOWSKI, THOMAS;PIGNOT, MARC;WINDHAB, NORBERT;BEHRENSDORF, HEIKE;MUTH, JOCHEN |
发明人 |
KAPPEL, ANDREAS;POLAKOWSKI, THOMAS;PIGNOT, MARC;WINDHAB, NORBERT;BEHRENSDORF, HEIKE;MUTH, JOCHEN |
分类号 |
G01N33/53;B01J19/00;C07H21/00;C07K14/195;C07K14/245;C12N9/00;C12N15/09;C12N15/52;C12Q;C12Q1/25;C12Q1/34;C12Q1/68;G01N33/566 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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