Process for the development of binding mini-proteins

摘要

The invention concerns a process for identifying proteins with a desired binding activity against a target, said process comprising (a) screening, for binding activity against said target, a population of genetic packages, each package displaying a potential binding domain, said population collectively displaying a plurality of different potential binding domains, said domains differing at one or more variable amino acid positions, each said potential binding domain being a micro-protein sequence of less than forty amino acids and having a single disulfide bond between a first amino acid position and a second amino acid position thereof, the amino acids at said first and second positions being invariant cysteines in the potential binding domains displayed by said population, and (b) identifying a protein having the desired binding activity against said target.