| 摘要 |
Isolated nucleic acids (N1) (all defined and given in the specification) from Corynebacterium glutamicumencoding metabolic pathway proteins (P1), provided that N1 does not consist of any F-designated genes (given in the specification), are new. Independent claims are also included for the following: (1) a nucleic acid molecule which encodes a naturally occurring allelic variant of P1; (2) a nucleic acid >= 50% homologous to N1; (3) a nucleic acid comprising >= 15 nucleotides of N1; (4) a nucleic acid hybridizing to any of those above; (5) a nucleic acid comprising any of the above sequences and a sequence encoding a heterologous polypeptide; (6) a vector comprising any of the nucleic acids above; (7) a host cell transformed with the vector of (6); (8) a method for producing P1 comprising culturing the cell of (7); (9) an isolated metabolic pathway polypeptide (P1) from Corynebacterium glutamicin; (10) allelic variants of P1; (11) a polypeptide with >= 50% homology to P1; (12) a method (M1) for producing a fine chemical comprising culturing a cell containing the vector of (6); (13) a method (M2) for producing a fine chemical comprising culturing a cell whose genomic DNA has been altered by the inclusion of any of the above nucleic acids; and (14) a method for diagnosing the presence or activity of C. diphtheriaein a subject comprising detecting the presence of N1 (selected from one of 1156 sequences given in the specification). |
