| 摘要 |
Bifunctional green fluorescent protein (GFP)-annexin fusion proteins combine the inherent strong visible fluorescent properties of GFPs with the anionic phospholipid binding specificity of annexins. Recombinant host cells, especially bacteria, are used to efficiently express the fusion proteins in high yield and soluble form, suitable for rapid, one-step affinity purification. Uses include of selective cellular and biochemical labeling, particularly anionic species, such as selectively labeling apoptotic cells.
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