DNA PCX-302 FRAGMENT ENCODING SYNTHESIS OF PENICILLIUM CANESCENS SECRETED ENDO-(1-4)-BETA-XYLANASE AND FUNGUS STRAIN PENICILLIUM CANESCENS AS PRODUCER OF SECRETED ENDO-(1-4)-BETA-XYLANASE

摘要

FIELD: genetic engineering, microbiology, biochemistry, enzymology. SUBSTANCE: invention relates to preparing DNA (PCX-302) fragment encoding synthesis of secreted endo-(1-4)-beta-xylanase based on isolation of genes of Penicillium canescens VKPM F-178 from phage library. Based on isolated DNA fragment the recombinant plasmid pPCXYLA is constructed and used for transformation of strain- -recipient of Penicillium canescens PCA 10 (nia D-). As result of screening of transformants the multicopy strain P. canescens XYL-23 (VKPM F-832) as producer of enzyme secreted form is obtained. In culturing obtained strain in pectin-containing medium with beet pulp it is possible to obtain up to 770 U activity of endo-(1-4)- beta-xylanase per 1 ml of cultural medium that exceeds by 6-7-fold the enzyme yield in using the strain- recipient. Invention can be used for preparing enzyme endo-xylanase in industrial scale. EFFECT: increased yield of enzyme. 2 cl, 2 dwg