| 摘要 |
Provided is a method of identifying tolerance in allograft recipients. It has been found that higher levels of circulating plasmacytoid DC (pDC2 cells), as compared to monocytoid (pDC1) cells (pDC2/pDC1) correlates with transplant tolerance. In one embodiment, the method includes determining the relative amounts of HLA-DR<+>lin<-> (CD3<->CD14<->CD19<->CD20<->)CD123<+>CD11c<->(pDC2) cells and HLA-DR<+>lin<-> CD123<lo>CD11c<+>(pDC1) cells in peripheral blood. The pDC1/pDC2 either can be determined from direct analysis of cells in a peripheral blood sample, or DC1 and DC2 cells can be cultured from a peripheral blood sample and the numbers of pDC1 and pDC2 cells in the peripheral blood sample can be estimated from the growth of the DC1 and DC2 cells in culture. |
