| 摘要 |
The present invention is related to a nucleic acid construct (1) to be incorporated in a double selection vector (2) able to transform a cell (3) of a specific organism, wherein-said construct (1) contains two different genes (10 and 11), each gene encoding a different toxic molecule (4 and 5) to a prokaryote cell, preferably to E. Coli, said genes (10 and 11) being disposed upstream and downstream a cassette sequence (8), or downstream and upstream site(s) for the insertion of a cassette sequence (8), and-said nucleic acid construct comprises specific sequence portions (12, 12') allowing inactivation of said genes (10 and 11).
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