发明名称 |
PCR method |
摘要 |
A method for balancing multiplexed PCR methods is provided. In the method, two or more sequential temporal PCR stages are used to effectively separate two or more PCR reactions in a single tube as an alternative to primer limiting to modulate the relative rate of production of a first amplicon by a first primer set and a second amplicon by a second primer set during the first and second amplification stages. Also provided are rapid RT-PCR methods that find particular use in intraoperative diagnoses and prognoses, for instance in diagnosing malignant esophageal adenocarcenoma by determining expression levels of carcinoembryonic antigen (CEA) in sentinel lymph nodes.
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申请公布号 |
US2003017482(A1) |
申请公布日期 |
2003.01.23 |
申请号 |
US20020090326 |
申请日期 |
2002.03.04 |
申请人 |
GODFREY TONY E.;LUKETICH JAMES D.;RAJA SIVA;KELLY LORI A.;FINKELSTEIN SYDNEY D. |
发明人 |
GODFREY TONY E.;LUKETICH JAMES D.;RAJA SIVA;KELLY LORI A.;FINKELSTEIN SYDNEY D. |
分类号 |
G01N33/53;C12N15/00;C12N15/09;C12P19/34;C12Q1/02;C12Q1/68;(IPC1-7):C12Q1/68 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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