| 摘要 |
<p>The present invention relates to a method for preparing Lipid II, which method comprises the steps of preparing a mixture of bacterial membranes, uridine diphosphate N-acetylmuramyl peptide (UDP-MurNAc), uridine diphosphate N-acetylglucosamine (GlcNAc), a chain of n prenyl residues, wherein n is at least 2, and optionally a detergent in a buffer; reacting the mixture thus obtained to prepare an n-Lipid II containing mixture, wherein n represents the amount of prenyl residues in the Lipid II molecule and wherein n is at least 2; and optionally further purifying the n-Lipid II containing mixture. Preferably n is selected from the range 2-25. Instead of bacterial membranes the reaction mixture may comprise isolated enzymes, such as MraY (undecaprenyl phosphate phospho-N-acetylmuramoyl-pentapeptide transferase).</p> |
