CANDIDATE REGION MISMATCH SCANNING FOR GENOTYPING AND MUTATION DETECTION

摘要

Use of the E. coli mismatch detection system, MutS, MutL and MutH, in PCR-based, automated, high-throughput genotyping and mutation detection of genomic DNA. Optimal sensitivity and signal-to-noise ratios are dependent upon monovalent cation concentration and MutL concentration. Strategies that can be easily adapted to automation for limiting the analysis to intersample heteroduplexes have been developed.