| 发明名称 |
DNA cloning method relying on the E. coli recE/recT recombination system |
| 摘要 |
The invention relates to methods for cloning DNA molecules using recE/recT-mediated homologous recombination mechanism between at least two DNA molecules where one DNA molecule is a circular or linear DNA molecule and the second DNA molecule is a circular DNA molecule, and the second DNA molecule contains two regions with sequence homology to the first DNA molecule. Competent cells and vectors are also described.
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| 申请公布号 |
US6509156(B1) |
申请公布日期 |
2003.01.21 |
| 申请号 |
US20000555510 |
申请日期 |
2000.06.05 |
| 申请人 |
EUROPAISCHES LABORATORIUM FUR MOLEKULAROIOLOGIE (EMBL) |
发明人 |
STEWART FRANCIS;ZHANG YOUMING;BUCHHOLZ FRANK |
| 分类号 |
C12N15/09;C12N1/21;C12N15/10;C12N15/90;C12R1/19;(IPC1-7):C12Q1/68;C12N1/20;C12N1/00;C12N15/00;C12N15/63;C12N15/70;C12N15/74 |
主分类号 |
C12N15/09 |
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