METHOD OF MULTIPLICATION OF STROMAL CELLS OF TESTICLES (TESTES), MALE SEXUAL GERMINAL CELLS, SPERMATOGONIA, SPERMATOCYTES AND SPERMATOZOA

摘要

FIELD: biology, biotechnology. SUBSTANCE: method of multiplication of stromal cells of testicles (testes), male sexual germinal cells, spermatogonia, spermatocytes and SPERMATOZOA involves their isolation, adaptation and culturing out of body and also integration (sewing in) genetic vector in their. Multiplication is carried out in bioreactors using nutrient media of more and more complex composition at each stage of culturing enriched with energetic, hormonal and enzyme components and by maintaining physical-chemical indices at optimal level at each stage. Method allows to obtain SPERMATOZOA with genetic vector of required directivity out of body in industrial scales. EFFECT: improved method of multiplication. 3 ex