In vivo protein-protein interaction mapping

摘要

The present invention is directed to the use of fluorescence resonance envery transfer (FRET) to screen for protein-protein interactions. More specifically, this invention provides a method to screen for and identify proteins that interact with a protein of interest. The method involves using DNA encoding the protein of interest tagged with one member of a fluorescent protein pair, and DNA sequences encoding a library of proteins to be screened, in which the proteins are tagged to the other member of the fluorescent pair. The protein of interest is co-expressed in cells with the library of proteins to be screened, such that single cell expresses the protein of interest and a single representative of the tagged library. This population of cells is screened by flourescence resonance energy transfer. Cells where interations have occurred are selected, allowing identification of the protein that interacts with the protein of interest.