发明名称 |
SUBSTRATES COMPRISING POLYNUCLEOTIDE MICROARRAYS |
摘要 |
A method of determining the relative amounts of individual polynucleotides in a complex mixture of different-sequence polynucleotides is disclosed. The polynucleotides, after fluorescent labeling, are contacted under hybridization conditions with an array of different DNA sequences disposed at discrete locations on a non-porous surface, at an array density of at least about 100 sequences/cm2, where the different DNA sequences in the array are effective to hybridize to individual polynucleotides in the mixture. The level of fluorescence associated with each array sequence provides a measure of its relative amount in the mixture.
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申请公布号 |
US2003012695(A1) |
申请公布日期 |
2003.01.16 |
申请号 |
US19980356322 |
申请日期 |
1998.11.24 |
申请人 |
SHALON TIDHAR DARI;BROWN PATRICK O. |
发明人 |
SHALON TIDHAR DARI;BROWN PATRICK O. |
分类号 |
B01J4/02;B01J19/00;B01L3/00;B01L3/02;C12Q1/68;C40B40/06;C40B40/10;C40B60/14;G01N1/28;G01N33/543;G01N35/00;G01N35/10;(IPC1-7):G01N15/06;C07H21/00 |
主分类号 |
B01J4/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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