发明名称 |
Purification method of luteinizing hormone and pharmaceutical preparation |
摘要 |
<p>A process for the purification of recombinant human Luteinizing Hormone (LH) from a sample of crude recombinant LH in the supernatant of CHO cells comprises the combined use of ion-exchange chromatography and reverse phase HPLC. The ion-exchange chromatography and the reverse phase HPLC are performed twice and the final use of a gel permeation column allows the purification from any residual traces of contaminants. The specific bioactivity of the highly purified LH obtained from the process is particularly high, amounting to about 25.000 IU/mg.</p> |
申请公布号 |
CZ20022856(A3) |
申请公布日期 |
2003.01.15 |
申请号 |
CZ20020002856 |
申请日期 |
2001.01.22 |
申请人 |
APPLIED RESEARCH SYSTEMS ARS HOLDING N. V. |
发明人 |
PARADISI GIANFRANCO;ROSSI MARA;SCAGLIA LAURA |
分类号 |
A61K47/26;A61K9/00;A61K9/19;A61K38/00;A61K38/24;A61P5/06;A61P5/34;C07K1/36;C07K14/59;(IPC1-7):C07K1/36;A61P5/24 |
主分类号 |
A61K47/26 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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