| 摘要 |
PROBLEM TO BE SOLVED: To provide a method for discriminating animal species by SINE(short interspersed element) method. SOLUTION: This method comprises the steps of preparing each genomic DNA library from at least one animal species to be discriminated, isolating each clone including an orthologous gene locus specifically inserted with a SINE family or subfamily in at least one of the animal species, amplifying by PCR the sequence of the gene locus for each of the animal species using a set of PCR primers annealing on the flanking sequences located on both ends of the SINE family or subfamily in the gene locus, and discriminating the animal species based on the presence/absence of a band indicating the presence of the gene locus by making a gel electrophoresis of the resultant PCR product. |
