| 摘要 |
PURPOSE: Aminopeptidase isolated from Bacillus licheniformis, a preparation process thereof, and a process for producing natural protein using the same enzyme are provided to easily mass-produce natural proteins including human growth hormone. CONSTITUTION: The aminopeptidase isolated from Bacillus licheniformis can remove methionine residue in the amino-terminal of peptides or proteins, and has the amino-terminal amino acid sequence of lysine - phenylalanine - serine - lysine - lysine - phenylalanine - asparagine - glutamic acid - asparagine - arginine, and the molecular weight of 43 to 47 kDa, wherein the amino acid sequence of the amino-terminal of peptides or proteins is methionine-X-proline; and X is phenylalanine. The process for preparing the aminopeptidase comprises the step of culturing Bacillus licheniformis, centrifuging the cultured medium, concentrating the supernatant, and purifying the aminopeptidase from the supernatant, wherein the Bacillus licheniformis is selected from Bacillus licheniformis ATCC 12759, Bacillus licheniformis KCTC 3058, Bacillus licheniformis KCTC 3045 and Bacillus licheniformis KCTC 1030. The process for producing peptides or proteins having the methionine free amino-terminal comprises treating peptides or proteins expressed by a recombinant method with the aminopeptidase isolated from Bacillus licheniformis, wherein the recombinant expressed protein is methionyl human growth hormone.
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