METHOD AND DEVICE FOR OBTAINING A SAMPLE WITH THREE-DIMENSIONAL MICROSCOPY

摘要

The invention concerns a method for obtaining a sample with three-dimensional microscopy, in particular a thick biological sample, and the fluorescence field emitted by said sample. The invention is characterised in that it comprises: a step of measuring and recording, which consists in measuring and recording sequentially and independently the interferometric signals obtained by digital holography and the fluorescence signals, derived from the sample; a processing step which consists in combining said interferometric signals obtained by digital holography and said thus recorded fluorescence signals so as to reconstruct clear three-dimensional images of the sample itself and of the fluorescence field emitted by the sample at a given time.