| 摘要 |
<p>The instant invention provides methods to determine the phosphorylation status or sulfation state of a polypeptide or a cell using mass spectrometry, especially ICP-MS. The invention also provides methods for identifying a substrate for a kinase using mass spectrometry. This invention further provides business method to conduct a drug discovery business. The invention further provides methods to determine the kinase activity of a peptide, ie., kinase, or the phosphatase activity of a peptide, ie., phosphatase. The invention further provides methods for identifying an inhibitor or an agonist of the kinase activity of a kinase, or an inhibitor or an agonist of the phosphatase activity of a phosphatase. (1) illustrates an autophosphorylation kinase assay determined by P and S centent.</p> |
