GENE TARGETING METHODS AND VECTORS

摘要

Methods and vectors are provided for the specific alteration of particular genetic loci in eukaryotic cells. One method includes the utilization of positive-positive selection (PPS) DNA vectors for the purpose of creating an d identifying cells which have vector sequences integrated into the host cell genome via site-specific homologous recombination. The procedure also comprises the utilization of sequences encoding in vivo detectable markers f or the identification of cells which have exogenous vector sequences integrated into the genome of the host cell, either via site-specific homologous recombination or nonhomologous recombination or insertion. The invention als o includes vectors for creating modifications in eukaryotic cells.