Verfahren zur fermentativen Herstellung von Pyruvat

摘要

The invention relates to a method for the fermentative production of pyruvate. The chemical methods for producing pyruvate known in prior art are technically complex and cost-intensive. The disadvantage of known biological methods is that the substrates are not converted 100 % into pyruvate and the product yield therefore suffers. The inventive method enables pyruvate to be produced microbially with a near 100 % conversion of the substrate. Increased production of pyruvate can be achieved by using micro-organisms that have been genetically modified, i.e. by the deletion or reduced expressed of nucleotide sequences that code for the pyruvate-dehydrogenase complex, pyruvate decarboxylase, pyruvate ferredoxin-oxidoreductase, pyruvate flavodoxin-oxidoreductase, pyruvate formiate-lyase, phosphoenolpyruvate synthetase and/or for pyruvate oxidase.