IMPROVED TRANSFORMATION AND REGENERATION OF WHEAT USING INCREASED COPPER LEVELS

摘要

A regeneration medium for use in wheat transformation which utilises copper at a concentration in a range from greater than 50 mM to less than or equal to 300 mM and a growth hormone at a concentration in a range from 0.05 to 10 mg l- 1 are disclosed. Growth hormone is added to the regeneration medium if wheat tissue or cell has not been treated with a growth hormone after callus induction and prior to using the wheat regeneration medium. A method for producing a transformed wheat plant using this regeneration medium is also disclosed.