发明名称 |
Isolation and purification of clostridium botulinum toxins |
摘要 |
A method for separating and purifying HA-positive progenitor toxin(s) (LL and/or L toxins) and HA-negative progenitor toxin (M toxin) from a Clostridium botulinum strain is provided. The method comprises applying a liquid containing both the HA-positive progenitor toxin(s) and the HA-negative progenitor toxin to a lactose column. Also provided is a method for separating and purifying neurotoxin (7S toxin) from HA-positive progenitor toxins, which comprises treating HA-positive progenitor toxins with an alkaline buffer and then applying the resulting liquid containing dissociated neurotoxin and non-toxic components to a lactose column. Activated pure HA-positive toxins (L and LL toxins) and neurotoxin are obtained by simple procedures. <IMAGE>
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申请公布号 |
EP1273593(A1) |
申请公布日期 |
2003.01.08 |
申请号 |
EP20020253624 |
申请日期 |
2002.05.23 |
申请人 |
OGUMA, KEIJI;EISAI CO., LTD. |
发明人 |
OGUMA, KEIJI |
分类号 |
C12P21/00;C07K1/22;C07K14/33;C12R1/145;(IPC1-7):C07K14/33 |
主分类号 |
C12P21/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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