Substrate peptides and assays for detecting and measuring proteolytic activity of serotype A neurotoxin from clostridium botulinum

摘要

Substrates for detecting and measuring the proteolytic activity of botulium type A neurotoxin in an assay are described. Detection is based on an increase in fluorescence due to hydrolysis of these internally quenched fluorescent peptide substrates by botulium type A neurotoxin. Several 13-15 amino acid peptides, derived from the substrate region of SNAP-25, have been constructed and analyzed for use in the assay.